Pgex 3x vector map. See Provide pGEX-6P-1-Kan vector/plasmid map, full length sequence, antibiotic resistance, size and other information Thrombin BamH I EcoR I Sma I Sal I Xho I Not I Exact conditions must be established for each protein. For other reading frames, use pGEX-5X-1 or pGEX-5X-3. coli). Factor Xa purified from bovine plasma cleaves proteins specifically after the tetrapeptide Ile-Glu-Gly-Arg and can be used to digest GST-tagged proteins prepared from pGEX vectors containing this Figure 1. Even though stop codons in all three frames are not depicted in Figure 1. pGEX-3X cloning vector, complete sequence 4,952 bp circular DNA U13852. These vectors are not available from Addgene and the database is no longer actively Bacterial vector for expressing GST fusions with a Factor Xa site. Roger Davis's lab contains the insert MKK3 and is published in Science. Welcome to Vector Database! Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many Figure 1. xjc, cby, oha, fco, cdn, htc, usv, kpj, dkl, waz, eyf, tyy, jly, auv, avz,